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Phage Australia sequence submission instructions
Please follow these instructions to submit your phage or bacterial genomes for accessioning and sequencing into the Phage Australia network.
Currently we only add items from partners within the network. Not a partner? Email us to join:
join@phageaustralia.org
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1. Download or Duplicate this spreadsheet. Then Fill in your Contact Details and your Sample Details.
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2. Make sure you adhere to the following requirements:
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ApplicationWGSmRNAseqTotal RNAseq
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Amount required1-500ng25-1000ng1-1000ng
Please provide maximum amount possible to minimize PCR bias
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Minimum volume required35ul30ul16ul
This will provide enough for library preparation and sample QC
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Recommended volume for submission70ul60ul32ul
This will provide enough for a repeat run if needed
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Delivery temperature4⁰C (for same day delivery) or -20⁰C-20-20
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3. Please label tubes with sample ID, date, and your initials. Include a printout of the form with your samples. Deliver samples to the address:

Westmead Institute for Medical Research
176 Hawkesbury Road, Westmead, NSW, 2145
Attention: Joey Lai
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4. Once you've put your samples in the mail, email this form to sequencing@phageaustralia.org
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Contact Details
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NameLabInstituteAddressContact NumberEmail
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Jessica SacherIredell LabWestmead Institute for Research
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Sample_idOrganismPhage_propagating_strainPhage_propagating_strain_idFasta_file_urlGenbank_accession
Dna_extraction_kit
Dna_concentrationNano_260_280Nano_260_230
Volume_submitted
Comments
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Internal ID used for phages or bacteria"Bacteriophage" or spell out bacterial speciesIf phage, the propagating strain. Helps us troubleshootIf phage, the internal ID of the propagating strain. Helps us troubleshoot(Optional) Add if previously sequenced; used for genome comparison projects; URL or instructions to download(Optional) Add if uploaded to Genbank; helps us compare and troubleshootIf custom kit or method, please add detailsNanodrop DNA concentration in DNA plate (ng/ul)Nanodrop 260 280Nanodrop 260 230in ul
(Optional) Additional concentration details, special requests, etc.
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phageIdNumberOneBacteriophageMycobacterium smegmatisMycobacterium smegmatis mc²155
https://phagesdb.org/media/fastas/Muddy.fasta
KF024728qiagen130.50.15 uL
Ready for sequencing run. Concentration in Qubit: 2.00 ng/ ul
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Pa01Pseudomonas aeruginosainvitrogen130.50.15 uL
Ready for sequencing run. Concentration in Qubit: 2.00 ng/ ul
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